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The DLC, which will launch for Switch this summer, was revealed during a Nintendo Direct presentation on Wednesday.
Its announcement trailer, which is viewable below, shows off new course Squeaky Clean Sprint.
It also reveals three more characters coming to the game – Petey Piranha, who originally appeared in Mario Kart: Double Dash, Wiggler from Mario Kart 7, and Kamek from Mario Kart Tour.
Priced at $24.99/£24.99, the Mario Kart 8 Deluxe Booster Course Pass will include 48 DLC tracks across six waves, all of which will be released by the end of 2023.
Players who subscribe to Nintendo Switch Online’s Expansion Pack receive access to the DLC as part of their subscription.
The fourth wave of Mario Kart 8 Deluxe DLC was released in March. It included classic tracks such as DK Summit from Wii and Mario Circuit from DS.
Mario Kart 8 Deluxe is Switch’s best-selling game. As of March 2023, it had sold 53.79 million copies, according to Nintendo.